期刊论文

中文

食品与机械

1003-5788

2019

35

1

32-40

10.13652/j.issn.1003-5788.2019.01.007

湖南省教育厅科研项目(编号:湘教39516C1496) 湖南省“2011”协同创新课题(编号:湘教通[2013]448号).

本校为其他机构

以3％葡聚糖硫酸钠(DSS)为诱导剂建立ICR小鼠结肠炎症模型,评估米糠多糖的抗炎功效与分子机理.采用病理组织分析、RT-qPCR和Western blotting等多种技术比较各组动物组织的病理改变和基因表达的差异.结果 发现:给予500 mg/(kg·d)米糠多糖灌胃能明显改善结肠炎症小鼠的整体健康状况,降低疾病活动指数分数,减轻小鼠结肠炎症组织的损伤,改善炎症相关生化指标.RT-qPCR分析发现,米糠多糖可使结肠组织炎症因子TNFα、IL-1β、Cox-2和iNOS等基因mRNA表达水平下调,Western blotting分析进一步证实了米糠多糖抑制炎症因子蛋白表达水平的功效,同时,米糠多糖也能抑制MAPK信号通路.结果 表明米糠多糖具有抗...

To assess the anti-inflammatory function and possible molecular mechanism of rice bran polysaccharide(RBP),DSS-induced colitis model was used in ICR mice.Pathological changes and gene expression were analyzed by histopathological analysis,RT-qPCR and Western blotting among of different experiment groups.The results showed that 500 mg/kg/day RBP could significantly improve the health status of mice and reduced colonic inflammation.RBP reduced the disease active index(DAI)score of DSS-induced mice and alleviated the damage of pathological tissue,...