期刊论文

中文

食品与机械

1003-5788

2013

29

6

143-146

基金项目：国家自然科学基金（编号：31070612）

本校为第一机构

探究油茶果皮多酚粗提物对DNA 氧化损伤的保护作用。采用单细胞凝胶电泳技术(SCGE)和琼脂糖凝胶电泳技术(AGE),分别研究不同浓度的油茶果皮多酚粗提物对H_2O_2诱导人脐静脉内皮细胞(HUVEC)DNA 损伤的保护作用,以及对APPH 损伤质粒pGL6DNA的保护作用。经SCGE分析显示:H_2O_2诱导细胞DNA严重损伤,在经50~1 000mg/L药物作用后,阴性对照组的细胞拖尾率和彗星尾长与阳性对照组相比均有显著的下降(P

To investigate the effect of Camellia pericarp polyphenol crude extracts on DNA damage. Using single cell gel electrophoresis and agarose gel electrophoresis, different concentrations of Camellia pericarp polyphenol crude extracts were investigated its protective effect on DNA damage of HUVEC induced by H2O2 ,as well as plas- mid pGL6 DNA induced by APPH. Result The analyzed results of SCGE showed that the cells induced by H2O2 was damaged serious- ly. Under the conditions of 50, 100, 500, 1 000 nag/L, the comet rate and comet tail length in ne...