Response surface analysis was applied to optimize the yeast enriching selenium fermentation conditions. Firstly, two-level facto rial design of Plackett Burman was used to evaluate the effect of six related factors on produced biomass and enriched selenium, and four main factors were screened out. Then the path of steepest ascent was taken to approach the optimal region of fermentation conditions, and the optimum levels of four most significantly factors were inoculation volume of 24.9%, Se dosage 18.7 mg/L, culture time 29.4 h, and medium loading rade of 68. 8 mL per250 mL flask. Under thecon...
