Hygroscopic behavior is an inherent characteristic of nanocellulose film which strongly affects its applications. In order to gain a better understanding of water adsorption, micro-Fourier transform infrared (FTIR) imaging was used to investigate the water adsorption in cellulose nanofiber film with a spatial resolution of 20 um. Four spectral peaks at 2905 cm−1, 1428 cm−1, 1371 cm−1, and 1317 cm−1 attributed to CH and CH2 groups were used to generate 2D micro-FTIR images of cellulose distribution, and the most intense peak at 3348 cm−1 wa...