期刊论文

中文

分子植物育种

1672-416X

2021

19

10

3208-3215

10.13271/j.mpb.019.003208

国家自然科学基金重点项目（31730016）资助；

本校为第一机构

本研究以油茶‘华硕’为试验材料,通过PCR克隆获得两个生长素响应SAUR （Small auxin upregulated RNA）基因家族的基因,命名为CoSAUR32和CoSAUR50。CoSAUR32基因开放阅读框（ORF）为372 bp,编码123个氨基酸;CoSAUR50基因开放阅读框（ORF）为318 bp,编码105个氨基酸。序列分析发现CoSAUR32和CoSAUR50蛋白均无信号肽与跨膜结构,在叶绿体和细胞核中均可表达。对其进行同源性比对及进化树分析发现,油茶两个SAUR与茶树SAUR亲缘关系最近。实时荧光定量PCR结果表明,CoSAUR32和CoSAUR50基因在自交授粉子房中36 h时的表达量显著高于异交子房,在自交授粉花柱中24 h时的表达量显著高于异交花柱。推测CoSAU...

In this study,Camellia oleifera’Huashuo’was used as experimental material.Two Samall auxin-up RNAs SAUR family genes were cloned by PCR and named CoSAUR32 and CoSAUR50.CoSAUR32 gene open reading frame(ORF)is 372 bp,encoding 123 amino acids,CoSAUR50 gene open reading frame(ORF)is 318 bp,encoding 105 amino acids.Sequence analysis showed that CoSAUR32 and CoSAUR50 proteins have no signal peptide and transmembrane structure,and can be expressed in chloroplasts and nucleus.Homology comparison and phylogenetic tree analysis showed that the two SAUR...