期刊论文

Cloning,Subcellular Localization and Expression Characteristics Analysis of LiAGL19 Gene in Crape Myrtle

中文

分子植物育种

1672-416X

2022

20

17

5605-5613

10.13271/j.mpb.020.005605

湖南省林业科技专项(XLK201912)资助。

本校为第一机构

AGL19是MADS-box基因家族的成员,在花芽分化与花器官发育过程中起重要作用.为了探索AGL19基因在紫薇花器官发育过程中的作用,以紫薇'湘韵'为试验材料,通过RT-PCR扩增技术,获得了紫薇AGL19基因序列,命名为LiA GL19基因(登录号为:MT364282).利用生物信息学手段对其进行生物信息学分析,并在烟草中进行亚细胞定位.此外,采用实时荧光定量PCR检测了目的基因在紫薇不同组织、不同时期花芽的表达量.结果表明,紫薇LiAGL19基因的编码区全长657 bp,编码218个氨基酸,是一个无信号肽、无跨膜区、亲水性的不稳定蛋白,与玫瑰木具有较高的相似性.亚细胞定位显示LiA GL19基因定位于细胞核中.实时荧光定量PCR分析结...

LiAGL19 is a member of the MADS-box gene family,and plays an important role in the process of flower bud differentiation and organ development of crape myrtle.In order to explore the AGL19 gene function of crape myrtle at floral organ development stage,the crape myrtle cultivar’Xiangyun’was selected as the experimental material,RT-PCR was employed to cloned a homologous gene of AGL19,named LiAGL19(GenBank Accession No.MT364282).Bioinformatics was utilized to analyze the protein,and subcellular localization was carried out in tobacco.In additi...