期刊论文

Callus Induction and Establishment of Cell Suspension Culture System for Curculigo orchiodes

中文

分子植物育种

1672-416X

2019

17

16

5383-5389

10.13271/j.mpb.017.005383

国家林业公益性行业科技专项项目(201304807)资助.

本校为第一机构

为初步建立仙茅(Curculigo orchiodes Gaertn)细胞悬浮培养体系。以仙茅幼叶为外植体,诱导产生愈伤组织后进行仙茅细胞悬浮培养研究。以MS为基本培养基,研究了植物生长调节剂对仙茅愈伤组织诱导和继代的影响,以及接种量,2,4-二氯苯氧乙酸(2,4-D),蔗糖,水解酪蛋白,2-氮吗啉乙烷磺酸(MES),L-脯氨酸(L-Pro)对仙茅细胞悬浮培养的影响。结果表明:仙茅愈伤组织诱导的最佳培养基为MS+6-BA 1.5 mg/L+ NAA 0.5 mg/L,所得的愈伤组织颜色淡黄,质地疏松,诱导率达100%;最佳继代培养基为MS+6-BA 2.0 mg/L+ NAA 0.5 mg/L,平均增殖倍数达10.8。仙茅细胞悬浮培养最佳培养基和培养条件为MS+2,4-D 0.5 mg/L+6-BA 2....

To preliminary establish the suspension culture system of C. orchiodes callus. Leaves were used as the explants to induce calli and study on the cell suspension of C. orchiodes. Using MS medium as the basic medium, effects of hormone on callus tissues induction and proliferation, as well as the inoculum amount Sucrose, Hydrolyzed casein, MES and L-Proline concentration on cell suspension culture of C. orchiodes were studied. The results showed that the optimal medium induction of C. orchiodes was MS+6-BA 1.5 mg/L+NAA 0.5 mg/L, the callus tissue...