期刊论文

Comparison of Gene Flow for Taxus chinensis var. mairei

中文

分子植物育种

1672-416X

2019

17

19

6269-6275

10.13271/j.mpb.017.006269

国家自然科学基金项目(31470666) 湖南省教育厅“十二五”重点学科资助项目(2011-76) 国家林业局重点按学科(林人发[2016]21号)共同资助.

本校为第一机构

为探索基因流的模式、时空变化规律及其对群体遗传结构的影响,本研究筛选了10个高多态性EST-SSR位点,对3个南方红豆杉群体进行了遗传变异和父本分析.根据群体间遗传分化系数计算历史基因流,利用父本分析结果估测雄性繁殖贡献率、有效花粉传播距离等现实基因流参数.结果 显示:10对EST-SSR引物共检测到80个等位基因,南方红豆杉群体的期望杂合度为0.785,群体间遗传分化系数为0.188,历史基因流为1.080.种群内亲代群体间、子代群体间基因流分别为1.140和0.923,亲子代群体间的固定指数存在显著差异.父本分析结果表明,3个自然授粉群体的雄性繁殖贡献率分别为37.50％、84.00％和18.91％,有效花粉传播距离...

In order to explore the modes of gene flow, space-time change law, and their effect on population structure, 10 highly polymorphic EST-SSR markers were screened for genetic variation and paternal analysis of three Taxus chinensis var. mairei populations. Historical gene flow were calculated according to the coefficient of genetic differentiation. Male reproductive contribution rate and effective pollen propagation distance were estimated by the results of paternal analysis. The results showed that 80 alleles were got from 10 EST-SSR primers. On the species level, expected heterozygosity were 0...