期刊论文

Preliminary Study on Curculigo orchiodes Gaertn Artificial Seeds

中文

分子植物育种

1672-416X

2017

15

8

3173-3178

10.13271/j.mpb.015.003173

国家林业公益性行业科技专项项目

本校为第一机构

本研究以仙茅幼叶为材料,采用不同的激素浓度诱导出愈伤组织,用愈伤组织诱导出不定芽。然后以不定芽为材料,用不同浓度的包裹基质和固化基质,制作一层膜人工种子和双层膜人工种子,测定它们的萌发率和失水率。结果表明:MS基本培养基中加入0.1 mg/L的2,4-D、1.5 mg/L的KT,3%的多菌灵和8 mg/L的柠檬酸,诱导愈伤组织效果最好,诱导率可达96%。以3%的海藻酸钠,MS基本培养基,0.3%多菌灵, 0.8 mg/L NAA,0.05 mg/L 6-BA作为人工胚乳。以2%的CaCl_2为固化剂,反应两次,第一次固化5 min,第二次固化10 min,制成的人工种子形态状况最好,失水率低且萌发率高。本研究采用双层膜方式,制作的人工种子在抗压和保水性...

In this study, Curculigo young leaves were used as material. We used different hormone concentrations to induce callus, and used callus to induce adventitious bud. Then adventitious buds were used as materials with different concentrations of encapsulation stroma and curing stroma, to make artificial seeds of one layer and double layer of membrane and measure the germination rate and the water loss rate. The result showed that the best effect of inducing callus was to use theMS medium with 0.1 mg/L of 2, 4-D, 1.5 mg/L of KT, 3% carbendazim and 8 mg/L of citric acid, the induction rate can reac...