期刊论文

Cloning and Prokaryotic Expression of a Fatty Acid β-Oxidation Multifunctional Protein Gene from Camellia oleifera

中文

分子植物育种

1672-416X

2016

14

6

1410-1420

10.13271/j.mpb.014.001410

国家自然科学基金(31070603) 湖南省自然科学基金(14JJ2104) 中南林业科技大学青年基金重点项目(QJ2011008A)共同资助

本校为第一机构

油茶是中国重要的木本食用油料树种,在油茶种子的发育过程中,涉及到一系列调控代谢过程的酶和基因,脂肪酸β氧化多功能蛋白(MFP)是β氧化系统上的一类酶,它完成催化β-氧化途径中的水合与脱氢过程。本研究以国审油茶品种‘华硕'种子为材料,在已构建的转录组和表达谱数据库的基础之上,采用 RACE技术,克隆到油茶脂肪酸β氧化多功能蛋白基因的cDNA全长序列,命名为CoMFP(GenBank登录号为KJ910342)。序列分析表明,该基因cDNA全长为2 541 bp,含有2 178 bp的开放读码框,编码725个氨基酸,分子量为79.033 3 kD,理论等电点pI为9.11,具有六个比较明显的跨膜区,不仅存有反式烯酰CoA水合酶(ECH)信号序列、3羟...

Camellia oleifera is a principal species of woody oil plants in China. There are many enzymes and genes to regulate the metabolism in seed development process. Fatty acid β-oxidation multifunctional protein (MFP) is a multifunctional protein playing a hydration and dehydrogenation role in fatty acid beta oxidation. In this study, state trial oil-tea variety ‘Huashuo'as material, a new full-length cDNA of MFP genes in C.oleifera were cloned by RACE and digitized transcriptome and expression profiling database. This gene was named CoMFP (GenBan...