期刊论文

中文

分子植物育种

1672-416X

2015

13

11

2500-2509

10.13271/j.mpb.013.002500

湖南省教育厅一般项目(13C1168) 中南林业科技大学校青年科学研究基金资助(QJ2012010B) 中南林业科技大学***项目(104-0119)共同资助 湖南省科技计划重点项目(2014FJ2004)

本校为第一机构

本研究以四季桂花瓣和叶为材料,采用RT-PCR和RACE技术获得四季桂DXPS基因cDNA全长,并用凝胶定量软件Quanlity-One对四季桂不同季节花瓣和叶片及不同花期花瓣的半定量PCR产物的凝胶图像进行分析。结果表明,四季桂DXPS基因cDNA全长2185 bp (GenBank登录号:KT099324),其中阅读框长1926 bp,编码641个氨基酸,含有保守功能结构域TPP-DXS、TPP-PYR-DXS-TK-like及Transketolase-C;半定量PCR结果分析表明,四季桂DXPS基因表达量在花朵盛开期最高,花蕾期次之,衰弱期最低;在花瓣中的表达量要明显高于同时期在叶片中的表达量;在花瓣中,12月份DXPS基因的相对表达量最高,3月份次之,在6月份和9月份DXPS相对表达量...

The full length c DNA sequence of DXPS(Deoxyoxylulose-5-phosphate synthase) gene was cloned from O. fragrans by using RT-PCR and RACE methods. The result showed that the full c DNA of DXPS gene in O.fragrans was 2 185 bp in length(Gen Bank accession No.KT099324), including 1 926 bp of open reading frame. It encoded a protein of 641 amino acid that contains several conserved domains, such as TPP-DXS, TPP-PYR-DXSTK-like and Transketolase-C. The result of semi-quantitative PCR indicated that the relative expression level of DXPS gene from high to low were the blooming stage the buding stage, and ...