期刊论文

The role of cell wall-degrading enzymes in the pathogenic process of Camellia oleifera disease caused by Colletotrichum gloeosporioides

中文

植物保护

0529-1542

2017

43

3

97-102

10.3969/j.issn.0529-1542.2017.03.016

(2012BAD19B0803):“十二五”国家科技支撑计划

本校为第一机构

为明确细胞壁降解酶在油茶炭疽病菌致病过程中的作用,本文研究了活体内外炭疽病菌产生的细胞壁降解酶活性及其对叶片的降解情况。结果表明,活体外以羧甲基纤维素钠(CMCNa)为诱导底物,羧甲基纤维素酶(Cx酶)和漆酶活性最高;以柑橘果胶为诱导底物,果胶酶活性最高;以油茶叶为诱导底物,纤维素酶、果胶酶和漆酶可产生较高活力;并且经5种诱导物诱导的酶液对叶片均有降解作用。发病叶片的各部位,以病健交界处细胞壁降解酶活性最高。接种4d后开始发病,其细胞壁降解酶活性迅速增强;6d后滤纸酶(FPA)、β-葡萄糖苷酶和漆酶活性达最大值,分别为4.53、7.44、1.21U/mg;而Cx酶和果胶酶在第8天时酶活性最高,分别为...

The research aims to estimate the role of cell wall-degrading enzymes(CWDEs) in the pathogenic process of Camellia oleifera anthracnose caused by Colletotrichum gloeosporioides.CWDEs produced by C.gloeosporioides both in vitro and in vivo were studied.The results showed that,with carboxymethyl cellulose sodium (CMCNa) as the inducer substrate,the activities of carboxymethyl cellulase and laccase were the highest,whereas citrus pectin was the best for pectinase.C.gloeosporioides could produce highly active cellulase,pectinase and laccase induced...