期刊论文

Wu, X.

中文

生态学报

1000-0933

2012

32

22

7247-7254

10.5846/stxb201110111491

(200909066):环保公益性行业科研专项 (2012BAC09B03):国家科技支撑计划项目 :国家林业局科技推广项目[2010]43号 (2012SK3167):湖南省科技计划项目 (2006180):湖南省环境科学重点学科建设项目

本校为第一且通讯机构

采用选择性平板培养基法和PCR-DGGE法比较研究了三级垂直潜流人工湿地污水处理系统中不同季节与单元之间根际微生物数量与多样性,结果表明,随着"秋-冬-春-夏"的季节的变化,2种方法测定的微生物数量都呈现"高-低-高"季节性变化现象,但选择性平板培养基法显示秋季数量最多,PCR-DGGE法显示夏季数量最多。从三级处理单元的1—3级,两种方法测定的微生物数量都呈现"低-高-低"的规律。除土样11外,其余相同季节的土样均出现聚集在同一个分支下。得到的4个差异序列(YP1—YP 4),经BLAST比较,分别找到同源性最近的已知类群,分别为肠杆菌属、弧菌、欧文氏菌、产酸克雷伯菌。BLASTX比较发现YP1与细菌代谢分解...

Selective plating medium and PCR-DGGE techniques were applied to analyze the seasonal changes in the number and diversity of micro-organisms in the rhizosphere of three sequenced cells in a vertical subsurface-flow wetland system constructed for municipal sewage treatment. The relationship between the community structures of the microorganisms and their functions in removal of the organic pollutants was also analyzed. The results obtained from the two applied methods showed that with the seasonal changes in the sequence of autumn- winter-spring-summer, the number of the micro-organisms display...