期刊论文、会议论文

Research Progress on Extraction, Purification and Content Determination of Plant Polysaccharides

中文

林产化学与工业

0253-2417

2009

B10

238-242

林业生物质化学与工程学术研讨会

林业生物质化学与工程学术研讨会论文集

2009-10-17

南京

中国林学会

“十一五”国家科技支撑计划资助（2009BADB1B03） 湖南省科技计划项目（2009NK3123）

本校为第一机构

综述了溶剂提取法、酶提取法、超声波与微波辅助提取法在植物多糖提取中的应用;分析了膜分离法、有机溶剂萃取法、柱层析法等分离纯化植物多糖方法的现状和前景;总结了苯酚-硫酸法、蒽酮-硫酸法、DNS（3,5-二硝基水杨酸）比色法、咔唑-硫酸比色法、高效凝胶渗透色谱法（HPGPC）等植物多糖的含量测定方法。植物多糖的分离纯化与含量分析,既是进一步研究其分子结构的基础,也为制备工业化产品提供参考。

The research progress on extraction, purification and content determination of plant polysaccharides were reviewed. Extraction methods such as solvent extraction, enzyme extraction, microwave and ultrasound-assisted extractions were summarized. The separation and purification methods were discussed, including membrane separation, organic solvent extraction, column chromatography, etc. The principle and application of content determination methods covering phenol-sulphuric acid method, anthrone-sulphuric acid method, 3,5-dinitrosalicylic acid （...