会议论文

Optimization the technology of extraction of protein derived from Camellia Oleifera Abel seed meal using response surface methodology

中文

2013

26-35

第三届中国林业学术大会

第三届中国林业学术大会论文集

2013-09-01

福州

中国林学会<&wdkj&>国家林业局<&wdkj&>福建省人民政府

本校为其他机构

在探明油茶粕蛋白等电点(pI=4.6)的基础上,采用碱溶酸沉法,研究了油茶粕蛋白的提取工艺.运用Design-Expert 7.1Trial统计分析软件,对油茶粕蛋白的碱提工艺条件进行了响应曲面法优化,获得了最佳的碱提工艺条件,即料液比1:25、浸提液pH值10.4、浸提时间2.42h、浸提温度49.2℃.在最佳的工艺条件下,蛋白提取率为65.83±2.45％(n=4),与模型预测值(64.97％)无显著差异(p＞0.05).

　　After determination the composition and isoelectric point value (pI=4.6) of the Camellia Oleifera Abel seed meal protein,using the methods of dissolving protein under alkaline condition and then settling protein under acidic condition,the extracting technology for protein was studied with the main aspect focused on the optimization of extracting technology through alkaline solution using response surface methodology (RSM) based on the software of Design-Expert 7.1 Trial.The optimal conditions of alkaline extracting for protein were as follo...