期刊论文

中文

昆虫学报

0454-6296

2011

54

6

729-733

10.16380/j.kcxb.2011.06.010

国家自然科学基金项目（30970421） 湖南省自然科学基金重点项目（06JJ20099） 湖南省教育厅重点科研项目（10A077） 湖南师范大学化学生物学及中药分析教育部重点实验室开放基金（KLCBTCMR2009-12）

本校为其他机构

【目的】探明苍耳Xanthium sibiricum活性物质——环氧苍耳素Ⅰ对菜青虫的作用机理。【方法】采用饲喂法处理4龄菜青虫Pieris rapae,测试从苍耳中分离提纯的环氧苍耳素Ⅰ（为倍半萜内酯类物质）对菜青虫中肠蛋白酶、淀粉酶和羧酸酯酶活性的影响。【结果】环氧苍耳素Ⅰ对蛋白酶活性的抑制最强,处理后12,24和48h,菜青虫中肠蛋白酶抑制率分别为20.95%,29.38%和50.06%;其次为淀粉酶,抑制率分别为11.89%,39.01%和31.92%。同时,中肠羧酸酯酶活性在处理后12h与对照之间无显著变化,24h时活性被显著抑制,而48h时活性却明显高于对照。【结论】环氧苍耳素Ⅰ对昆虫中肠消化酶活性的抑制,可能是引起昆虫表现取...

[Aim] This study aims to reveal the mechanism of epoxyxanthatin Ⅰ （sesquiterpe lactone from Xanthium sibiricum） on Pieris rapae larvae.[Methods] The activity of midgut protease,amylase and carboxylesterase in P.rapae larvae were investigated by feeding to 4th larvae of P.rapae.[Results] Epoxyxanthatin Ⅰ showed the most obvious inhibition on midgut protease,whose activities were inhibited 20.95%,29.38% and 50.06% at 12 h,24 h and 48 h after treatment respectively,while it showed the less obvious inhibition on midgut amylase,whose activities ...