Our study showed that the hemolymph juvenile hormone (JH) titer exhibited a high‐amplitude daily rhythm in long‐winged female Velarifictorus aspersus (Orthoptera: Gryllidae), a species showing wing dimorphism. The crickets utilized glycogen and trehalose as flight fuel. The high‐amplitude daily rhythm of the JH titer was correlated with biosynthesis of flight energy substances and flight ability; that is, an elevated JH titer promoted the biosynthesis of flight energy substances, resulting in the improvement of flight capacity. Abstract The ...