期刊论文

Screening and Expression Analysis of Related Genes Based on Transcriptome Sequencing of Ginkgo Flower Buds at Three Differentiation Stages

Wang, Y.

中文

园艺学报

0513-353X

2018

45

8

1479-1490

10.16420/j.issn.0513-353x.2018-0023

国家自然科学基金项目（31570682） 国家重点研发计划项目（2017YFD0600701）

本校为第一机构

鉴定银杏花芽分化调控的关键基因,揭示银杏花芽分化调控的主要分子机制,为缩短银杏童期和选育银杏早花品种提供理论指导.本研究中采用高通量测序技术对银杏花芽分化3个时期(花芽未分化期、花芽分化始期、花芽分化盛期)的样品进行转录组测序,并分析数字表达谱,筛选开花调控相关基因并进行荧光定量PCR(RT-qPCR)表达验证.转录组测序共产生27.52 Gb原始数据,注释到8大功能数据库(GO、COG、KEGG、KOG、NR、Pfam、Swiss-Prot、eggNOG)上的unigene总数为35 179个.通过GO分类和KEGG Pathway富集性分析,将unigene分别归于55个GO类别和126个代谢途径.差异表达基因分析显示,花芽未分化期较花芽分化始期有2 ...

This study identified of Ginkgo biloba flower bud differentiation key genes，to reveal the main molecular mechanism of ginkgo flower bud differentiation regulation，and provide theoretical guidance for shortening the juvenile phase and breeding early flowering varieties. High-throughput sequencing technology was used to sequence the transcriptomes of three different stages of flower bud differentiation，and digital expression profiles were analyzed to screen ginkgo flowering regulatory related genes. Genes were verified by qPCR. A total of 27.5...