期刊论文

Extraction of Tannins from Wild Persimmon Leaf with Semi-Bionic and Antioxidation Analysis

中文

湖北农业科学

0439-8114

2015

01

148-151

10.14088/j.cnki.issn0439-8114.2015.01.038

湖南省科技计划项目（2013GK3184）；

本校为第一机构

采用半仿生法提取野柿子树叶单宁,对其提取工艺进行优化,并对其抗氧化能力进行检测。结果表明,采用半仿生法能够提高单宁对OH·、DPPH·和ABTS＋·等自由基的清除能力,其IC50值均低于水浴加热法及酶法提取。正交试验优化得到的最佳工艺条件为提取温度40℃、提取时间60 min、溶剂体积分数50%、3次提取液p H分别为2.0、7.8和9.0以及原料粒度120目。在此条件下,单宁提取量可达206.8mg/g,远高于水浴加热法及酶法提取的样品。

Tannin was extracted from wild persimmon leaf using Semi-Bionic approach. The technology of extraction was optimized and the antioxdation activity was detected. The results showed that the Semi-Bionic approach improved the scavenging activity of tannins on radicals, including OH·, DPPH·, and ABTS＋·. The half maximal inhibitory concentration values were lower than that of the water-bath heating and enzymic method. Experimental results of orthogonal optimization showed that the yield of tannin reached 206.8 mg/g, higher than that of the sampl...