期刊论文

中文

计算机与应用化学

1001-4160

2010

27

5

577-581

10.3969/j.issn.1001-4160.2010.05.004

国家“863计划”项目（2006AA102341,2008AA100801） 湖南省重大科技专项（2007FJ1007） 长沙理工大学***基金项目.

本校为第一机构

α-葡萄糖苷酶是生物体中一类非常重要的酶,在初级代谢及复合糖的生物合成与加工上具有重要作用. α-葡萄糖苷酶在自然界广泛分布,种类繁多,但目前对其分子结构和催化机制的了解还非常有限,因此预测并比较不同来源的α-葡萄糖苷酶结构的共性和个性具有重要的科学价值. 本文从Swiss-Prot数据库中选取了4种典型微生物来源的α-葡萄糖苷酶,以EMBOSS和Clustal W在线工具比较了其基本参数和蛋白质序列,以Modeller软件预测了其三级结构,并用Castp服务器预测了它们的活性位点. 根据其系统发育树和三维结构的相似性,这4种α-葡萄糖苷酶可分为AglA和YihQ,Bce33和Mal12 2组;构成它们活性位点pocket的氨基酸...

The α-glucosidases, which are widely distributed in the nature but with ess information about their molecular structure and catalytic mechanism. They are a particularly important subset of these enzymes in live organisms, both in primary metabolism and in glycoconjugate biosynthesis and processing. In this paper, the structure and active site of four α-glucosidases from different microbial origin was predicted and contrasted, using the soft Modeller and the web servers of Castp. The results showed that they can be divided into two groups, Agl...