期刊论文

中文

植物遗传资源学报

1672-1810

2022

23

5

1521-1535

10.13430/j.cnki.jpgr.20220412004

国家自然科学基金重点项目（31730016）；

本校为其他机构

植物自交不亲和性（self-incompatibility，SI）中SSI和GSI主要受S位点控制。油茶属于后期自交不亲和（LSI）植物，油茶自交不亲和性已成为油茶产业发展的制约因素。为探究油茶自交不亲和分子机制，本研究以课题组前期研究的油茶雌蕊转录组数据和已完成基因注释的油茶良种‘华硕’全基因组数据中获得的5条S-RNase（CoS-RNase）基因组序列为基础设计引物，从10个油茶品种的叶片DNA中扩增CoS-RNase外显子，结合雌蕊CoS-RNase的cDNA全长克隆预测CoS-RNase氨基酸多态性位点，共获得28条CoS-RNase等位基因。基因结构分析显示CoS-RNases基因包含4个外显子和3个内含子；cDNA全长1141bp，ORF 717bp，编码...

SSI and GSI in self-incompatibility(SI)plant are mainly controlled by S locus.Camellia oleifera Abel.belongs to Late-acting self-incompatibility(LSI)plant,and its self-incompatibility has become a limiting factor to the development of Camellia oleifera Abel.industry.To investigate the molecular mechanism of LSI in Camellia oleifera Abel.,we designed primers for five S-RNase(CoS-RNase)genes sequences derived from the preliminary study of the annotated genome-wide data of elite variety called Huashuo and the pistil transcriptomic data of camellia oleifera Abel..By PCR amplification of CoS-RNases...