期刊论文

Characterization and Development of EST-SSR Markers from Transcriptome Datasets of Firmiana danxiaensis H.H.Hsue & H.S.Kiu

中文

植物遗传资源学报

1672-1810

2019

20

5

1325-1333

10.13430/j.cnki.jpgr.20190121001

:野生动植物保护与自然保护区管理项目 2016ZWZY06:韶关市野生动植物保护办公室项目

本校为第一机构

以丹霞梧桐转录组序列为基础,开发丹霞梧桐EST-SSR标记位点,并利用其对丹霞梧桐群体进行遗传多样性分析,评价丹霞梧桐群体的遗传多样性水平,为丹霞梧桐的合理利用和保护提供依据。应用Primer 3.0、Gen Al Ex6.3、FSTAT和MS-tools等软件进行引物设计和相关遗传参数分析。17858个SSR位点分布于79920个unigene序列,SSR位点密度为1/4.78 kb。SSR重复基序中,三核苷酸(43.64%)为优势核苷酸,其次为四核苷酸(23.52%)和二核苷酸(15.54%)。三核苷酸和二核苷酸优势重复基序分别为AAG/CTT和AG/CT。以16个样本为材料,从设计的73对引物中共筛选出16对多态性EST-SSR引物,多态性信息含量(PIC)的平均值为0.546,所...

The microsatellite markers in Firmiana danxiaensis H.H.Hsue & H.S. Kiu were developed by taking use of the transcriptome dataset.These markers were subjected for analyzing the genetic diversity of extant populations to provide basis for rational utilization and the protection of Firmiana danxiaensis H.H.Hsue & H.S. Kiu. Primer 3.0,GenAlEx6.3,FSTAT and MS-tools were deployed to explore primers and analyze the genetic parameters.As a result,17858 SSRs were identified from 79920 unigenes with a frequency of 1/4.78 kb.Trinucleotide,tetranucleotide and dinucleotide accounted for 43.64%,23.52% and 1...