期刊论文

中文

中南林业科技大学学报

1673-923X

2024

44

01

175-184

10.14067/j.cnki.1673-923x.2024.01.017

XLK202108-6:湖南省林业科技创新***培养科研项目 2023JJ30358:湖南省自然科学基金项目 XLKY202212:湖南林业科技创新项目 XLKY202317:湖南林业科技创新项目

本校为第一机构

[目的]组装华中樱叶绿体基因组并进行序列特征分析和分子标记初步鉴定.[方法]分别应用SPAdes、prodigal v2.6.3、hmmer v3.1b2及aragorn v1.2.38软件进行华中樱叶绿体基因组组装、基因编码区注释、rRNA和tRNA预测.应用OGDRAW软件进行叶绿体基因组图谱绘制.运用DNAMAN软件对 26 个樱亚属物种和华中樱的叶绿体基因组序列进行多重比对,人工检索华中樱特有的SNP和InDel位点.应用Codon W1.4.2和EMBOSS的cusp软件分析叶绿体基因组中基因的相对同义密码子使用度.[结果]华中樱叶绿体基因组大小为158 019 bp,其中小单拷贝区(Small single copy,SSC)、大单拷贝区(Large single copy,LSC)和反向重复区(Inve...

[Objective]To assemble the chloroplast genome of Prunus conradinae and carry out sequence analysis and preliminary identification of molecular markers.[Method]SPAdes,prodigal v2.6.3,hmmer v3.1b2 and aragorn v1.2.38 software were used to assemble the chloroplast genome,annotate the gene coding region,and predict rRNA and tRNA.The genome map of chloroplast was drawn by OGDRAW.Multiple alignment of chloroplast genome sequences of P.conradinae and 26 subgenus Cerasus species was carried out using DNAMAN software,and the SNP and InDel sites unique t...