期刊论文

Effects of exogenous Ca~(2+) on pollen germination of Camellia oleifera under NaCl stress

中文

中南林业科技大学学报

1673-923X

2021

41

6

91-98

10.14067/j.cnki.1673-923x.2021.06.010

国家自然科学基金重点项目“油茶自交不亲和分子机制解析”（31730016）；

本校为第一机构

【目的】探讨NaCl对油茶花粉生活力、花粉萌发和花粉管生长的影响,以及Ca~(2+)是否有缓解作用,旨在探索在盐渍化地区引种油茶的可能性,为油茶的引种和栽培提供参考资料。【方法】以油茶‘华硕'花粉为试验材料,采用MTT染色法和花粉液体培养法,研究NaCl胁迫以及在NaCl胁迫环境中添加Ca~(2+)后对油茶花粉生活力、萌发率和花粉管生长的影响。【结果】1)NaCl对油茶花粉生活力、花粉萌发和花粉管伸长均有抑制作用,且抑制效果随NaCl浓度的提高而增强,而高浓度NaCl可完全抑制花粉萌发。20 mM NaCl的抑制效果显著(P < 0.05),此时花粉生活力和花粉萌发率分别为36.22%和42.84%,分别是CK的49.97%和57.33%,花...

【Objecctive】To explore the effects of NaCl on pollen viability, pollen germination and pollen tube growth of Camellia oleifera, and whether Ca~(2+) can alleviate it, in order to explore the possibility of introducing C. oleifera to salinized areas and provide reference for its introduction and cultivation. 【Method】 Using C. oleifera ‘Huashuo' pollen as experimental material, the effects of NaCl stress and the addition of Ca~(2+) in the NaCl stress environment on the pollen viability, germination rate and pollen tube growth of C. oleifera w...