期刊论文

Transcriptome analysis of Paulownia fortunei root in response to lead-zinc mine stress

中文

中南林业科技大学学报

1673-923X

2020

40

8

128-138

10.14067/j.cnki.1673-923x.2020.08.016

国家环保公益性项目(201509037) 湖南省环保科研项目(湘财建指2016-59) 湖南省研究生科研创新项目(CX2016B341)。

本校为第一机构

【目的】白花泡桐Paulownia fortunei (Seem.) Hemsl.为中国南方重要的尾矿造林树种。为了揭示泡桐根系受重金属胁迫后的转录调控机制,明确泡桐根系响应重金属胁迫的相关基因和重要通路。【方法】以生长在铅锌矿、南方红壤的白花泡桐根为实验材料,进行高通量测序,将组装的基因序列在Nr、Nt、Pfam、KOG/COG、Swiss- Prot、KEGG和GO数据库中进行基因功能注释,同时对在红壤、铅锌矿中差异表达的泡桐根部基因进行了分析。 【结果】1)经过测序共得到366 701 960条reads序列,总碱基数为53.90 Gb,GC平均含量达44.58%。将测序所得reads再经Trinity软件组装后得到126 061条基因序列,其中96 133条序列获得...

【Objective】P. fortunei is an important tailings afforestation tree species in southern China. In order to reveal the transcriptional regulation mechanism of P. fortunei roots under heavy metal stress, and to clarify the related genes and important pathways of P. fortunei roots system in response to heavy metal stress.【Method】High-throughput sequencing was carried out with the roots of P. fortunei grown in lead-zinc mine, southern red soil as experimental materials. The assembled Unigene sequences were annotated in Nr, Nt, Pfam, KOG/COG, Swi...