期刊论文

Improve lupeol biosynthesis in Arabidopsis through overexpression of AtLUP1

中文

中南林业科技大学学报

1673-923X

2019

39

6

107-113

10.14067/j.cnki.1673-923x.2019.06.016

湖南省教育厅优秀青年项目(17B282) 湖南省研发计划项目(2017NK2252).

本校为第一机构

羽扇豆醇是一种存在于多种果蔬及中草药中的五环三萜类化合物,具有抗炎症、抗氧化、抗癌等药理作用。羽扇豆醇合酶（Lupeol synthase,LUP）是羽扇豆醇合成途径中的关键酶。前期研究显示,编码该酶的基因在原核和酵母表达体系中均难以获得有生物活性的蛋白产物。本研究以拟南芥为受体,通过超量表达拟南芥羽扇豆醇合酶1（At LUP1）基因,分析利用植物细胞生产羽扇豆醇的可行性。使用气相色谱GC检测At LUP1超量表达拟南芥植株中的羽扇豆醇含量,发现48号植株中羽扇豆醇含量为0.915 5 mg·g-1,显著高于野生型。本研究证明超量表达At LUP1基因可以在拟南芥中促进羽扇豆醇的合成,为羽扇豆醇的工业化生产提...

Lupeol, a pentacyclic triterpenoid compound, has been found in diverse fruits, vegetables and medicinal plants. It has pharmacological effects of anti-inflammatory, anti-oxidation and anti-cancer. Lupeol synthase is a key enzyme for the biosynthesis of lupeol. Previous studies showed that it is difficult to obtain lupeol products with bioactivity either in prokaryotic or yeast expression systems. In this study, Arabidopsis was usedas a receptor for AtLUP1 overexpression to analyze the feasibility of enhancing lupeol production in plant cells. The contents of lupeol in AtLUP1 overexpressing str...