期刊论文

Effects of hormon on somatic embryogenesis from different provenances of Cinnamomum camphora

中文

中南林业科技大学学报

1673-923X

2018

38

12

115-120,135

10.14067/j.cnki.1673-923x.2018.12.016

“十三五”国家重点研发计划项目“樟树等高效培育技术研究”（2016YFD06006005）；

本校为第一机构

基因研究依赖于植株再生,香樟离体培养植株再生以体胚发生为主。以岳阳、郴州、醴陵、长沙、怀化、娄底樟树Cinnamomum camphora未成熟合子胚作为外植体,研究不同激素在体胚发生过程中对体胚及愈伤组织诱导、芽分化和生根的影响,目的在于为湖南省不同种源樟树体胚发生建立基础,完善樟树植株再生体系。结果表明:适合岳阳、怀化樟树诱导体胚发生的最适培养基为MS+1 mg·L~(-1) 6-BA+0.1 mg·L~(-1) 2,4-D,其余种源均为MS+1 mg·L~(-1) 6-BA+0.3 mg·L~(-1) 2,4-D;岳阳、怀化、娄底种源樟树芽分化最佳培养基为MS+5 mg·L~(-1) 6-BA+ 0.1 mg·L~(-1) NAA,醴陵和长沙种源樟树为MS+3 mg·L~(-1) 6-BA+...

Gene research depended on plant regeneration, in vitro plantlet regeneration of Cinnamomum camphora was mainly embryogenesis. Explants from the immature zygotic embryos of Yueyang, Chenzhou, Liling, Huaihua, Loudi, Changsha, research different hormones in the process of embryo effects on induction, somatic embryo callus and bud differentiation and rooting, this study established the basis for the emergence of different species of camphor tree somatic embryos in Hunan Province, and improved the plant regeneration system of camphor tree. The resu...