期刊论文

中文

中南林业科技大学学报

1673-923X

2016

36

12

129-134,139

10.14067/j.cnki.1673-923x.2016.12.022

国家林业行业公益性项目“珍贵树种钩栗良种选育及栽培关键技术研究”（201204405）；

本校为第一机构

利用ISSR分子标记对钩栗Castanopsis tibetana Hance 9个野生居群进行了遗传多样性分析。利用100条引物分别对111份钩栗基因组DNA进行PCR扩增,共扩增出158条清晰条带,其中多态性条带141条,多态性条带百分率(P)平均为89.01%。钩栗9个居群的Nei's基因多样性指数、Shannon's信息指数分别为0.332 3、 0.492 0;总种群基因多样度(Ht)、各种群基因多样度分别为(Hs)0.410 8、0.334 6,表明钩栗的遗传多样性较高。9居群的基因分化系数Gst为0.185 5,占总遗传多样性的81.45%。分子方差分析表明,80.21%的遗传差异存在于群体内,19.79%的遗传差异存在于群体间。基因流为2.196 0。用NTSYS软件计算出9居群111材料...

The genetic diversity of nine Castanopsis tibetana Hance populations were analyzed using inter-simple sequence repeat （ISSR） markers. 10 ISSR primers were screened out from 100 primers from UBC for amplifying 111 genomic DNA of Castanopsis tibetana using ISSR-PCR. 158 intensive bands were amplified, among which there were 141 polymorphie bands. The polymorphie loci percentage （P） was 89.01%. The Nei＇ s genetic diversity index, Shannon＇ s information, number of total genetic diversity index, genetic diversity index are 0.3323, 0.4920, 0.41...