期刊论文

中文

中南林业科技大学学报

1673-923X

2015

35

4

40-45

10.14067/j.cnki.1673-923x.2015.04.007

国家林业公益性行业科研专项（201204610） 湖南省科技计划重点项目（2014FJ2004）

本校为第一机构

红檵木是极具观赏价值的常绿灌木。以红檵木的叶片和茎段为材料,通过优化愈伤组织诱导与增殖,不定芽诱导、增殖与生根的培养基与激素配比,进行红檵木高效再生体系研究。结果表明,红檵木外植体最佳消毒方案为两次升汞消毒法:0.1%HgCl_2两次消毒,各3min;最佳愈伤组织诱导与增殖的培养基配方分别为:MS+6-BA1.0mg/L+NAA1.7mg/L、MS+6-BA1.0mg/L+IBA0.06mg/L;首次使用AgNO_3,获得最佳不定芽诱导培养基配方:MS+6-BA1.5mg/L+NAA0.0mg/L+AgNO_31mg/L;不定芽增殖最佳培养基配方为:MS+6-BA1.0mg/L+IBA0.05mg/L+Vc5.0mg/L;最佳不定芽生根方案为:选择2.1~3.0cm的不定芽,保留2~4叶片,接种至1/2MS+IBA4.5mg/L培...

Loropetalum chinense var. rubrum is a kind of evergreen shrub which has great ornamental value. Using stems and leaves as explant, the high-efficient regeneration system of L. chinense var. rubrum was researched by optimizing the medium type and hormone combination in induction and multiplication of callus, induction and rooting of adventitious buds. The results show that the optimal sterilizing conditions was: disinfected twice with 0.1% HgCl2 sterilization each lasted 3 minutes; the optimal mediums for callus induction and multiplication were: MS+6-BA 1.0 mg/L+NAA1.7 mg/L and MS + 6-BA 1.0 m...