The screening of SSR primers for forestry is a heavy research which is very time consuming and consume intensive experimental materials.By employing genomic DNA pooling technology,a method of mixing several genomic DNA templates equivalently as the single template for PCR,was used to quickly screen SSR primers,which will significantly reduce the experimental workload and cost.Genomic DNA pooling constructed by equivalently mixing 6 Eucalyptus genomic DNA templates was used to screen 150 pairs of EST-SSR primers.The analytical results show that comparing with conventional screening method,the g...
