期刊论文

中文

中南林业科技大学学报

1673-923X

2012

32

8

81-85

10.14067/j.cnki.1673-923x.2012.08.024

湖南省省级科技计划（专项计划）项目“耐贮保鲜高抗性转基因猕猴桃的研究和开发”（2008JT3008） 湖南省高校产学研合作示范基地开放项目（2011jsjk003）

本校为第一机构

为建立猕猴桃基因功能研究技术平台并通过生物技术改良猕猴桃,选用米良一号叶片和茎为外植体,通过优化培养基,建立了适合转化的高效再生系统,通过农杆菌介导将ACC(1-氨基环丙烷-1-羧酸)氧化酶基因成功转化米良一号,构建了根癌农杆菌介导米良一号遗传转化体系。结果表明,TD培养基为合适的愈伤诱导培养基,诱导率达100%,愈伤经3次继代培养转入分化培养基,分化率为90%。共获得29株潮霉素抗性植株,随机挑选14株经PCR检测,其中10株检测到ACC氧化酶基因目的条带,阳性植株占71.4%。

In order to establish the technology platform for function analysis of genes and genetic improvement ofkiwifruit through biotechnology, the leaves and stems of Miliang-1 plants were used as explants. An efficient regeneration system was established for gene transformation by optimizing the culture medium. By using Agrobacterium-mediated method, transgenic plants containing the ACO （1-Aminocyclopropane-l-carboxylate oxidase） gene fragment were successfully obtained. The results show that the TD medium was the efficent medium for callus inducti...