The isolation methods of high-quality genomic DNA of dry Moss plants such as rock samples and years-preserved specimen is one of the key difficulties restricting its researches of molecular biology. Six main methods including common CTAB, high-salt CTAB, improved CTAB, Genome Kit, SDS and fast extraction were used to isolate genomic DNA of dry Moss specimen for many years. The results show that the improved CTAB is the optimal method to isolateMoss specimen genomic DNA, whose total yield is higher and impurity is lesser. The ISSR analytical results show that the genomic DNA isolated by improve...
