期刊论文

中文

中南林业科技大学学报

1673-923X

2012

32

6

118-122,127

10.14067/j.cnki.1673-923x.2012.06.005

国家公益性行业科研专项经费项目（编号201004001） 湖南省科学技术厅科技计划重点项目（编号201lWK2001）：常德市科学技术局技术研究与开发资金项目（编号2011GK06）

本校为第一机构

利用羧甲基纤维素钠培养法、刚果红染色法、滤纸崩解法和胞外纤维素酶活性测定法,从湖北神农架保护区不同区域采集的森林腐质土壤中分离筛选得到一株高产纤维素酶的真菌B-5。结合菌落形态观察、显微镜观察和ITS序列同源性分析,确定该菌株为镰刀菌属Fusarium,与木贼镰刀菌Fusarium equiseti相似度达99%。滤纸崩解实验显示该菌72 h对滤纸具有明显崩解能力,失重率达37.9%。以羧甲基纤维素钠为碳源,蛋白胨为氮源,28℃,180 r/min,培养5 d,初始酶活为FPA酶活(FPase)2.09 IU/mL,CMC酶活(CMCase)5.20 IU/mL。通过产酶条件优化,确定稻草为唯一碳源,初始pH为6.1,氮源浓度为1.0%,碳源浓度为2.5%,鼠李糖脂添...

An effective cellulase-producing fungus named B-5 was isolated from the primary forest soil in different regions of the protected area in Shennongjia, Hubei, China, by using the methods of CMC-Na culture medium, Congo red staining, disintegration of filter paper and measurement of enzymatic activity of cellulose. The strain was identified as Fusarium by observing its colony morphology and microscopic characteristics as well as ITS sequence homology analysis, having the similarity of 99% with Fusarium equiseti. The filter disintegration experiments show that the fungus had strong capacity of fi...