期刊论文

中文

中南林业科技大学学报

1673-923X

2011

31

3

54-59

10.14067/j.cnki.1673-923x.2011.03.024

国家自然科学基金项目（30471417）；

本校为第一机构

以银杏雌树成熟叶为材料构建cDNA文库,随机挑取2 324个克隆进行5′端测序,并将所得序列与核酸数据库进行同源性比较.结果显示:银杏叶光合作用基因ESTs为97条,占能量代谢相关的基因ESTs总数的75%;与光反应相关的基因主要有PSII捕光叶绿素a/b结合蛋白,放氧复合体蛋白,质体蓝素(PC),PSI捕光叶绿素a/b结合蛋白,PSI反应中心亚单位,铁硫蛋白,铁氧蛋白酶,ATP合成酶等;与暗反应相关的基因主要有核酮糖二磷酸羧化酶/加氧酶(RuBisCO),核酮糖二磷酸羧化酶/加氧酶激酶RuBisCO activase(RA),甘油醛--3--磷酸脱氢酶,核桐糖--5--磷酸异构酶,丙糖磷酸异构酶等;RuBisCO基因表达相当丰富,与落叶松Larix laricinaRu...

Based on 5′-sequencing of the 2 324 clones from cDNA library of female Gingko mature leaves,a homology comparison the obtained sequence to genes from the GenBank at NCBI was carried out.Analysis of the Ginkgo biloba EST library revealed a number of ESTs with high BLAST similarity to genes with known roles in higher plant photosynthesis process.These genes consisted of those involved in the light-reaction and dark-reaction of the plant photosynthesis,and the ESTs was 97.75% of the total ESTs from energy metabolization.The former was similar to ...