期刊论文

中文

中南林业科技大学学报

1673-923X

2010

30

12

6-10

10.14067/j.cnki.1673-923x.2010.12.019

教育部博士点基金项目（20060538004） “十一五”国家科技支撑计划课题（2006BAD24B0301） 湖南省教育厅重点项目（07A077） 湖南省教育厅优秀青年项目（09B112）资助

本校为第一机构

利用ISSR分子标记技术,对湖南城步马尾松种子园的4个种源区的11 7个无性系的马尾松遗传多样性进行了研究.采用了10个ISSR引物扩增共获得了218个标记位点,其中有210个为多态位点,多态位点比率为 96.33%.不同种源区多态位点百分率在88.99%~90.37%之间.其中两广种源区的多态位点百分率最高,达 90.37%,说明其种源内遗传变异水平在4个种源区中是最高的.总的Shannon多样性指数为0.503 0.Nei,s遗传多样性指数为0.334 7.各个种源区的遗传一致度的变化范围在0.968 9~0.981 2之间,各群体间的基因分化系数为0.039 3,基因流为12.221 904个种源区间Nei's遗传距离UPGMA聚类分析结果表明,遗传距离 (0.0190)最小...

Genetic diversity of 117 clones in 4 populations of Pinus massoniana Lamb.seed orchard in Chengbu,Hunan Province was studied using ISSR markers.Ten ISSR primers,selected from 100 UBC primers,were applied to amplify all clones and a total of 218 markers were obtained.Among them,210 are polymorphic loci and the rate of polymorphic loci is 96.33%.The percentage of polymorphic loci between populations shared a range of 88.99%~90.37% and the highest（90.37%） was between Guangdong and Guangxi populations,which explained its ability adapts to circums...