期刊论文

中文

中南林业科技大学学报

1673-923X

2010

30

10

68-71,77

10.14067/j.cnki.1673-923x.2010.10.017

国家“十一五”科技支撑项目（2009BADB1B02 2006BAD18B0204） 国家自然科学基金项目（30371184） 湖南省自然科学基金项目（07JJ3070）

本校为第一机构

为研究油茶种子成熟调控蛋白基因的功能,以pCAMBIA1304质粒为载体,根据油茶种子成熟调控蛋白基因的cDNA序列设计了小干扰RNA（Si RNA）序列,构建了油茶种子成熟调控蛋白基因小干扰RNA植物表达载体pCAMBIA1304-si RNA,并借助农杆菌介导叶盘法将pCAMBIA1304-si RNA转化到野生型烟草中,获得了稳定表达的转基因烟草苗。为下一步的油茶种子成熟调控蛋白基因的功能鉴定奠定了基础。

In order to study the regulation of Camellia oleifera seed ripening-regulated protein gene function,pCAMBIA1304 plasmid was designed as the vector.Small interfering RNA（SiRNA） was sequence according to the Camellia seed ripening-regulated protein gene cDNA sequence,and Camellia oleifera seed ripening-regulated small interfering RNA regulation of gene expression in plants vector pCAMBIA1304-siRNA was constructed,and pCAMBIA1304-siRNA were transformed into wild-type tobacco by Agrobacterium-mediated leaf disc transformation,thus the transgenic ...