期刊论文

中文

中南林业科技大学学报

1673-923X

2010

30

2

1-9

10.14067/j.cnki.1673-923x.2010.02.010

国家“十一五”科技支撑项目（2009BADBIB02,2006BAD18B0204） 国家自然科学基金项目（30371184） 湖南省自然科学基金项目（07JJ3070）

本校为第一机构

以油茶'湘林1号'品种的近成熟种子为材料,采用简并PCR、RACE、交错PCR等技术,获得了油茶乙酰辅酶A羧化酶BC亚基基因的全长cDNA克隆.该基因cDNA序列全长1 901 bp,含有一个1 599 bp的ORF,编码533个氨基酸残基.BC蛋白的等电点pI为6.88,分子量为58 509.3 U,含两个跨膜结构域,是一个不稳定的非分泌蛋白.模体搜索结果表明在BC上具有N-糖基化位点、蛋白激酶C磷酸化位点、ATP结合位点等多个功能结构域.同源建模的模型中发现12个α-螺旋,整个BC蛋白为一个内凹的结构,该基因已登录到GenBank,并被命名为CO-bc.

With the near-matured seeds of‘Xianglin No. 1＇ of Camellia oleifera as material to extract total RNA,the whole length eDNA of BC gene that is a subunit of ACCase from Camellia oleifera was obtained by means of the techniques such as degenerate PCR, RACE and the overlap extension PCR. The full length of eDNA sequence of BC gene is 1 901 bp, and has a 1 599 bp ORF coding 533 amino acids. The pI value of BC protein is 6.88, the molecular mass of the protein is 58 509. 3 u, the protein bears two trans-membrane domain, has no signal peptide, and i...