Stems from Pinus rnassoniana seedlings were cultured on different kinds of media and hormones. Results show that the best basal medium for inducting callus is improved GD, namely, improved GD+4 mg · L-12,4-D + 2 mg · L-16-BA+500 mg · L-1CH +30 mg · L-lsucrose+8 mg · L-1agar; and that the average diameter of inducted callus can grow to 5.8 mm after 20 d dark culture. This study lays some scientific foundation for the differen...