期刊论文

中文

中南林业科技大学学报

1673-923X

2007

27

1

9-13

10.3969/j.issn.1673-923X.2007.01.002

国家自然科学基金（30371184） 湖南省自然科学基金（05JJ40125）.

本校为第一机构

查尔酮合酶和查尔酮异构酶是类黄酮代谢与色素苷代谢的关键酶.以油茶近成熟种子cDNA文库和EST文库为材料,通过分子克隆方法鉴定了1条查尔酮合酶基因全长cDNA和1条查尔酮异构酶全长cDNA.结果表明:油茶查尔酮合酶基因的cDNA含1 479 bp,编码412个氨基酸,为目前最长的查尔酮合酶,与其它物种的查尔酮合酶具有极高的相似性,在进化上高度同源;油茶查尔酮异构酶基因的cDNA含899 bp,编码206个氨基酸,与茶的查尔酮异构酶基因高度同源,但与其它物种的相似性较低.

Chalcone synthase （CHS） and chalcone isomerase （CHI） are the key enzymes controlling the metabolism of flavonoid and anthocyanin. In this paper, 1 full-length cDNA of CHS and 1 full-length cDNA of CHI were separated from the cDNA library and EST library of Camellia oleifera by the method of molecular cloning. The cDNA of C. oleifera CHS contains 1 479 bp for 412 amino acids, which is so far the longest CHS and displays high evolution homology with those other species for very high similarity. The cDNA of C. oleifera CHI contains 899 bp for ...