期刊论文

Analysis of Development-related Genes in the EST Library of the Nearly Matured Seeds of Camellia oleifera

中文

中南林学院学报

Journal of Central South Forestry University

1673-923X

2005

25

4

17-23

10.14067/j.cnki.1673-923x.2005.04.004

国家自然科学基金资助课题“油茶EST文库构建及重要基因的分离克隆”(编号:30371184)； 教育部骨干教师资助课题“油茶cDNA文库构建及探针筛选”项目的部分内容.；

本校为第一机构

以油茶优良无性系湘林1号和湘林4号近成熟种子为材料构建cDNA文库,随机挑取2327个克隆进行3′端测序,并将所得序列与核酸数据库进行同源性比较.发现16种58条与油茶种子胚胎发育及种子成熟相关的基因序列,这些序列与核酸数据库中其他物种相关序列有很高或较高的同源性.其中,脱落酸应答蛋白、成熟控制蛋白、翻译控制肿瘤蛋白、胚胎特化蛋白、乙烯应答因子包含域蛋白等基因序列为高丰度表达;种子成熟蛋白、细胞分化蛋白、乙烯应答因子、脱水素等编码基因属中等丰度表达;而成熟相关蛋白、胚珠发育蛋白、伸展蛋白、生长控制因子、休眠相关蛋白、衰老相关蛋白和脱水应答蛋白等基因只检测到1条序列,属低...

To investigate the gene expression during the transforming peak of seed oil in Camellia oleifera, a eDNA library of nearly matured seeds of two clones, i, e, XL-1 and XL-4, was constructed. 2 327 clones were randomly selected for sequencing from the 3＇- end, and between these sequences and nucleic acid database a homology comparison was made , Its result shows that 58 ESTs are highly homological to genes of ovule development and seed maturation of other plant species in the database of NCBI and dbEST, and that decoding genes of abscisic acid r...