The unsaturated fatty acid of plants is correlative to its cold-resistance. The method of the quantitative analysis of fatty acids in the leaves of Eucalyptus camaldulensis was studied by using CHCl3-CH3OH mixed solvent distilling, pentadecanoic acid internal standard, KOH-CH3OH-BF3 esterification, silica-gel decoloration and gas chromatography determination. The results show that the myristic acid was not determined, the palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid and eleosetearic acid were determined. The relative response factor of each fatty acid was 1. 000, 0....