期刊论文

Wang, YH

英文

Journal of Analytical Chemistry

1061-9348

2019

74

5

505-512

10.1134/S1061934819050101

ACKNOWLEDGMENTS This work was supported in part by the Research Foundation of Education Bureau of Hunan Province, China (16A227), Hunan Provincial Natural Science Foundation of China (2018JJ3869), Science and Technology Innovation Foundation of Graduate Students in Hunan Province (CX2016B3). National Natural Science Foundation of China (21305164).

本校为第一且通讯机构

Abstract: A simple electrochemical method for detecting glutathione reductase was developed in this work. It was observed that the impedance of electrode increased obviously after the electrode modification by glutathione, and the value of impedance was closely related to the reduced glutathione (GSH) concentration. Based on the fact that glutathione reductase (GR) could catalyze oxidized glutathione (GSSG) to GSH rapidly in the presence of NADPH (β-nicotinamide adenine dinucleotide 2'-phosphate reduced), and as GSH was immobilized on the gold...