期刊论文

Zanthoxylum dissitum ZdICL gene cloning expression and its expression changes during seed germination

Sun, J.-K.

中文

中草药

0253-2670

2018

49

19

4628-4635

10.7501/j.issn.0253-2670.2018.19.025

国家自然科学基金资助项目（31370612） 湖南省高校科技成果产业化繁育项目（15CY011）

本校为第一且通讯机构

目的克隆表达蚬壳花椒种子萌发过程中油脂代谢关键调控基因,研究该基因在蚬壳花椒种子外源激素赤霉素（GA）促萌发下调控蚬壳花椒种子人工萌发分子机制。方法根据蚬壳花椒转录组测序结果,筛选克隆油脂代谢关键基因,并利用原核表达体系表达纯化目的蛋白,对其编码产物进行生物信息学分析和预测。种子萌发实验分为清水对照组与GA促萌发实验组,设定0、6、12、24、48 h为萌发取样点,相对荧光定量分析该基因表达量变化。结果通过克隆该基因片段及序列分析,确定该基因编码异柠檬酸裂解酶（ICL）,将其命名为ZdICL。该基因开放阅读框全长为1 728 bp,编码产物包含575个氨基酸。构建原核表达载体pET-28a-Zd...

Objective To clone and express the key genes regulating oil metabolism during seed germination of Zanthoxylum dissitum, and investigate the molecular mechanism of the gene regulating artificial seed germination of Z. dissitum under the stimulation of exogenous hormone gibberellin (GA) of its seeds. Methods According to the transcriptome sequencing results of Z. dissitum, the key genes related to lipid metabolism were screened and cloned, the target protein was expressed and purified by prokaryotic expression system, and the encoded products wer...