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基于转录组测序的银杏类黄酮生物合成关键基因表达分析

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成果类型:
期刊论文
论文标题(英文):
Transcriptome sequencing analysis of gene expression of flavonoid biosynthesis in Ginkgo biloba
作者:
刘伟;王俊燚;李萌;董金金;何崇单;...
通讯作者:
Wang, Y.-Q.
作者机构:
[王俊燚; 李萌] Key Laboratory of Non-wood Forest Nurturing and Protection of National Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
[董金金; 何崇单] Hunan Provincial Key Laboratory for Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
[汪贵斌; 郁万文] College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu 210037, China
[刘伟; 王义强] Key Laboratory of Non-wood Forest Nurturing and Protection of National Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan 410004, China, Hunan Provincial Key Laboratory for Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
通讯机构:
[Wang, Y.-Q.] K
Key Laboratory of Non-wood Forest Nurturing and Protection of National Ministry of Education, China
语种:
中文
关键词:
银杏;转录组;类黄酮;黄酮醇
关键词(英文):
Illumina HiSeq 2000
期刊:
中草药
ISSN:
0253-2670
年:
2018
卷:
49
期:
23
页码:
5633-5639
基金类别:
“十三五”国家重点研发计划专项(2017YFD0600701) 国家自然科学基金项目(31570682).
机构署名:
本校为第一且通讯机构
院系归属:
林学院
生命科学与技术学院
摘要:
目的对银杏进行转录组测序,分析银杏生长发育不同时期类黄酮生物合成关键基因的表达。方法以银杏幼年树和成年树不同时期叶片作为供试材料,利用Illumina HiSeq 2000进行转录组测序,对Unigene进行功能注释,分析银杏类黄酮生物合成关键基因的表达特征。结果转录组测序共获得43 073条Unigene,其中35 179条被注释,基因差异表达(DEG)筛选得到5 117个基因。通过对类黄酮合成相关KEGG途径进行分析,筛选出50条候选基因。分析50条候选基因的表达规律,发现类黄酮合成关键基因均在银杏幼叶中高表达,但在成年树与幼年树同时期叶片之间表达差异不显著。分析与类黄酮合成关系密切的13个基因发现,其中肉桂酸4-羟...
摘要(英文):
Objective The transcriptome sequencing was used to analyze the expression of key genes of flavonoid biosynthesis in different stages of growth and development of Ginkgo biloba. Methods The leaves of young trees and adult trees of G. biloba in different periods were used as the test material. Transcriptome sequencing analysis was carried out by using Illumina HiSeq 2000, and analyses of gene functional annotation of Unigene and expression characteristics of key genes for biosynthesis of G. biloba flavonoids were also performed. Results A total o...

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