期刊论文

Ma, Y.-Z.

中文

中草药

0253-2670

2014

45

12

1769-1774

10.7501/j.issn.0253-2670.2014.12.023

林业公益性行业科研专项经费“金银花、凹叶厚朴新品种创制及利用技术研究”（201104023） 湖南省教育厅重点项目（2012A0146）

本校为第一且通讯机构

目的 建立凹叶厚朴Magnolia officinalis subsp.biloba快速繁殖体系.方法 以选育的优质凹叶厚朴种胚为初始外植体,采用单因素及正交试验筛选不同基本培养基,不同植物调节剂(6-BA、NAA、IBA、IAA),获取最适种胚启动培养基,丛芽增殖培养基和生根培养基.结果 适宜种胚萌发的启动培养基为B5+6-BA 0.5 mg/L+NAA 0.1 mg/L,出芽率达87.0％;适合丛芽增殖的培养基为MS+6-BA 2.5 mg/L+NAA 0.5 mg/L,增殖系数可达6.2;适合生根的培养基为1/2 MS+NAA 0.5mg/L,30d生根率可达84％.结论 建立了凹叶厚朴快速繁殖体系,为凹叶厚朴优质种苗的工厂化育苗奠定了基础.

Objective: To establish a rapid propagation system of Magnolia officinalis subsp. biloba. Methods: Using peeling seeds of M. officinalis subsp. biloba as the initial explants, different media and various combinations of plant growth regulators (6-BA, NAA, IBA, and IAA) on the seeds germination, the seedlings subculture, and rooting culture were studied by single factor test and orthogonal test. Results: The best culture medium of seeds germination was B5 + 6-BA 0.5 mg/L + NAA 0.1 mg/L, and the budding percentage was 87.0%; The effective medium for cluster buds-inducing and subculture was MS + ...