期刊论文

Down-regulation of decorin expression in chondronecrosis of Kaschin-Beck disease

中文

中华地方病学杂志

Chinese Jouranl of Endemiology

1672-1810

2016

16

10

725-730

10.3760/cma.j.issn.2095-4255.2016.10.006

国家自然科学基金(81273006、30872187);教育部留学回国人员启动基金(11回国基金01);National Natural Science Foundation of China(81273006,30872187);The Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry(11return funds 01)

本校为其他机构

目的 观察大骨节病(KBD)儿童手指关节软骨和低硒条件下T-2毒素中毒大鼠膝关节软骨核心蛋白聚糖(Decorin)的表达,以及T-2毒素和硒对培养软骨细胞Decorin表达的影响,分析Decorin与KBD软骨坏死的相关性.方法 以5例尸检KBD儿童(KBD组)和5例因车祸死亡或畸形手术儿童(对照组)手指关节软骨为研究对象,采用免疫组织化学染色法检测儿童手指关节软骨Decorin的表达情况.选取雄性健康SD大鼠32只,按体质量采用随机数字表法分为常规组、低硒组,每组16只,常规组饲料硒含量为101.5 μg/kg,低硒组饲料硒含量为1.1 μg/kg,30 d后常规组又分为常规组和常规+T-2组,低硒组分为低硒组和低硒+T-2组,每组8只,T-2毒素(10...

Objective To investigate the expression of Decorin in Kaschin-Beck disease （KBD） cartilage as well as in a KBD model of T-2 toxin under selenium deficient conditions and in human chondrocyte in vitro in order to determine a possible mechanism underlying KBD.Methods Immunohistochemical staining of Decorin was performed on sections of human phalanges of hands from normal （samples from 5 normal children,traffic accident or operation） and KBD children （samples from children with KBD）,and 32 Sprague-Dawley rats were divided into two groups acc...