期刊论文

中文

中药材

1001-4454

2016

39

6

1357-1360

10.13863/j.issn1001-4454.2016.06.039

(2012BAC01B07-2):国家科技支撑计划 (201104044):国家林业公益行业科研专项 (CX2014A06):中南林业科技大学研究生创新项目 (CX2015B284):湖南省研究生创新项目

本校为第一机构

目的:利用响应面法优化樟叶精油的亚临界萃取技术并考察其抑菌活性。方法:在单因素试验的基础上,以樟叶精油得率为响应值,利用Box-Behnken中心组合试验优选亚临界萃取条件。采用固相微萃取与气相色谱-质谱法(GC-MS)结合对樟叶精油的成分进行分析,通过峰面积归一法计算各成分的相对百分含量。利用滤纸片琼脂扩散法和肉汤稀释法测定樟叶精油对4种供试菌的抑菌活性。结果:在最优的萃取条件(萃取时间30 min,萃取温度40 ℃,解析温度65℃)下樟叶精油得率为3.54%,这与模型预测值3.56%相匹配。GC-MS鉴定出47个化合物,其中相对百分含量超过1%的有12种成分,主要为桉油精(24. 74%)、4-亚甲基-1-(1-甲基乙基...

Objective : To determine the optimum extract condition of essential oil from dry Cinnamomum camphora leaves and to study its antibacterial activity. Methods : The essential oil was extracted by subcritical fluid,and analyzed by response surface methodology based on single factor test. The chemical compositions of essential oil were analyzed by GC-MS,and the components were quantitatively determined by normalization method. The agar disc diffusion and dilution broth method were used to determine antibacterial activity. Results :The extraction ra...