期刊论文

中文

北方园艺

1001-0009

2016

5

119-123

10.11937/bfyy.201605032

长沙市科技计划资助项目（kl307012-21）；

本校为第一机构

以白檀未成熟胚为试材,以改良MS为培养基,研究了白檀未成熟胚的器官发生和植株再生.结果表明:改良MS(mMS)能够很好的诱导愈伤组织,在添加了0.2 mg/L 6-BA+0.1 mg/L NAA的培养基中,诱导率高达92.5％;胚性愈伤组织分化最佳培养基为mMS+0.25 mg/L 6-BA+0.15 mg/L NAA,分化率为72.4％;分化出的胚性愈伤组织在空白mMS培养基上继代培养15 d,76.6％的组织分化出芽,再转入1/2mMS+ 1.5％蔗糖培养基上培养20 d,芽长至1.5 cm.

Taking immature zygotic embryos of Symplocos paniculata as materials, using modified MS medium, the organogenesis and regeneration were researched. The results showed that the mMS medium could induce callus greatly, the highest induction rate 92. 5% was obtained on the medium with 0. 2 mg/L 6-BA＋0. 1 mg/L NAA. 72. 4% of calluses initiated green bud-like structures when they were cultured on raMS plus 0. 25 mg/L 6-BA＋0. 15 mg/L NAA. The calluses with green bud-like structures were subcultured onto raMS medium without plant growth regulators fo...