期刊论文

Genetic diversity and fingerprint construction of varieties of Hydrangea macrophylla by ISSR markers

中文

中南林业科技大学学报

1673-923X

2016

36

12

115-120

10.14067/j.cnki.1673-923x.2016.12.020

国家林业局948项目“绣球花植物新品种及繁育技术引进”（2013-4-35） 湖南省科技厅重点研发项目：绣球花新优品种培育及开发利用（2016NK2143） 湖南省教育厅一般项目“绣球花品种遗传多样性的ISSR研究及指纹图谱构建”（15C1433）

本校为第一机构

利用ISSR-PCR分子标记技术对23个绣球花品种进行遗传多样性研究,从100条引物中筛选出10条引物对供试材料基因组总DNA进行PCR扩增,共扩增出条带123条,其中多态性条带102条,占82.93%,具有较高的多态性。经PopGen32软件包分析,23个绣球花品种的平均有效等位基因数为1.493 7,平均Nei's基因多样性指数为0.290 7,平均Shannon信息指数为0.435 7,遗传距离介于0 ~ 0.769 1之间,遗传一致度介于0.463 4 ~ 1.000 0之间,具有广泛的遗传多样性,梦幻蓝和史欧尼10条引物的扩增结果一致,二者可能为同一个品种。UPGMA聚类将23个品种分成2类,聚类结果与叶片特征一致。引物UBC855可以将23个绣球花品种完全区分,依此建...

ISSR-PCR was used to detect the genetic diversity and relationship of 23 varieties of Hydrangea macrophylla. ISSR fingerprinting amplified by 10 ISSR primers which were selected from 100 ISSR primers revealed a total number of 123 unambiguous bands, of which 102 ones were polymorphic and the polymorphism frequency was 82.93%. As analyzed by PopGen32, the mean effective number of alleles of 23 varieties of Hydrangea macrophylla, the mean Nei＇s genetic diversity index is 0.2907, the mean Shannon information index is 0.435 7, the genetic distance...