Aqueous enzymatic extraction (AEE) was applied to obtaining the free oil from castor (Ricinus communis) seeds in this study. Individual enzymes and enzymatic combinations were screened, and the reaction conditions of AEE were modified using response surface methodology. And the effects of solvent extraction (SE) and AEE on the oil's physicochemical properties were compared. The enzyme of nutrase was found to be the best one in extracting free oil compare to other enzymes (cellulase, hemicellase, pectinase, and amylase), and the highest oil recovering rate of 77.53% was achieved under optimal e...